Terms of Use & Licence

This website is free and open to all users and there is no login requirement. All web tools available at digitalgens.org — including DigitalGens — are free to use for everyone: academic, research, clinical and commercial users alike, without registration, payment, or usage limits.

The software is released as free and open-source software under the GNU General Public License, version 3 (GPL-3.0). The complete source code (HTML, JavaScript, CSS) is publicly available on GitHub. Under this licence you are free to:

• Use the tools online or offline, for any purpose, including commercial use.
• Study the source code and verify how every result is computed.
• Modify the code and create derivative works.
• Redistribute the original or your modified version, provided you keep it under the GPL-3.0, retain the copyright notice, and make the corresponding source code available.

No prior permission from the author is required for any of the above. The full, legally binding text is the GPL-3.0 itself.

github.com/rkalendar/PCRtools Full licence text (LICENSE.txt) GPL-3.0 at gnu.org

Copyright © Ruslan Kalendar. Licensed under the GNU GPL v3.0.

The full implementation including all primer-design algorithms, scoring functions, thermodynamic parameters, and the user interface is available in the public GitHub repository. This enables:

• Independent verification of all results by reviewers and users.
• Local deployment for personal or institutional use — clone the repository and open sites/index.html in any modern browser to run the entire toolbox offline, without any external server.
• Issue reporting and feature requests via the GitHub issue tracker.
• Reuse of individual modules in third-party projects, under the terms of the GPL-3.0 and without prior arrangement.

All computations run locally in the user's browser; user sequences are never transmitted to any server. See the Data & Privacy section of the documentation for details.

For a full list of recommended citations, see the References & Citation page.

All tools run entirely in the user's web browser using client-side JavaScript. No installation, plug-ins, or server-side processing is required. Tested on:

• Google Chrome 148+
• Mozilla Firefox 151+
• Microsoft Edge 148+
• Apple Safari 26+

Works on Windows, macOS, Linux, ChromeOS, and modern mobile browsers. A minimum of 4 GB of RAM is recommended for genome-scale repeat analysis and large multiplex panel design.

The tools are provided "as is" without warranty of any kind, express or implied, including but not limited to the warranties of merchantability, fitness for a particular purpose, and non-infringement. The author makes no representations about the suitability of these tools for any particular purpose.

The author shall not be held liable for any direct, indirect, incidental, or consequential damages arising from the use of these tools.

• All computations are performed locally in your browser (client-side JavaScript); your sequences are never sent to our servers.
• Optional retrieval functions (NCBI/Ensembl) send requests directly to those external services only when you click"Retrieve".
• If you work with sensitive or proprietary sequences, prefer manual FASTA upload and use an offline/local copy of the tools where applicable.

Modification and redistribution need no permission — see the licence. For scientific questions, bug reports or collaboration:

• Bug reports & feature requests: GitHub Issues — the preferred channel, and the fastest.
• Email: ruslan.kalendar@digitalgens.org — for scientific questions, collaboration, or anything that does not belong in a public issue.
• Author: Prof. Ruslan Kalendar, Helsinki Institute of Life Science (HiLIFE), University of Helsinki — contact details on the University research portal.

Reporting a problem: nothing about your session reaches us — the tools run entirely in your browser and keep no logs — so please say which tool, which browser, and paste the input you used. Without that there is nothing to reproduce.